published in:Biotechnology Techniques 3,
173-178.
language: English
M. Dubrovsky1, V. Petera1,
B.Sikyta2, H. Hegerova2
1Institute of Physics of Atmosphere, Czech. Acad. Sci.
50008 Hradec Kralove, Czechoslovakia
2Institute of Microbiology, Czech. Acad. Sci. 14220
Prague, Czechoslovakia
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(I am sorry for the low qulity of the copy, but the paper was
published as a camera-ready manuscript, and I had not a laser
printer in those ancient times. Moreover, my scanner is no Rolls
Royce... But you can read it!)
SUMMARY
The ice nucleation activity of the bacterium P.syringae
CCM 4073 was determined by a drop freezing technique and
expressed through the relative freezing nucleus spectrum -
fraction of nucleation active cells vs. temperature. The spectrum
was found to be independent of cell concentration, quite stable
within periods of the order of 1 h, and "step-wise",
the steps being most conspicuous at -5C and -9C.