published in:Biotechnology Techniques 3, 173-178.
language: English


MEASUREMENT OF THE ICE NUCLEATION ACTIVITY OF PSEUDOMONAS SYRINGAE CCM 4073

M. Dubrovsky1, V. Petera1, B.Sikyta2, H. Hegerova2
1
Institute of Physics of Atmosphere, Czech. Acad. Sci. 50008 Hradec Kralove, Czechoslovakia
2Institute of Microbiology, Czech. Acad. Sci. 14220 Prague, Czechoslovakia

 


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SUMMARY
The ice nucleation activity of the bacterium P.syringae CCM 4073 was determined by a drop freezing technique and expressed through the relative freezing nucleus spectrum - fraction of nucleation active cells vs. temperature. The spectrum was found to be independent of cell concentration, quite stable within periods of the order of 1 h, and "step-wise", the steps being most conspicuous at -5C and -9C.